Publications

2024

Toyoda, M., Fukuda, T., Fujimoto, R., Kawakami, K., Hayashi, C., Nakao, Y., Watanabe, Y., Aoki, T., Shida, M., Sanui, T., Taguchi, M., Yamamichi, K., Okabe, A., Okada, T., Oka, K., Nakayama, K., Nishimura, F., & Kajioka, S.
" Scaffold-free bone-like 3D structure established through osteogenic differentiation from human gingiva-derived stem cells.
Biochemistry and Biophysics Reports,Volume 38, July 2024, 101656

 

2023

Skylar-Scott M, Declercq H, and Nakayama K
" Special Issue: Biofabrication with Spheroid and Organoid Materials"
Acta Biomaterialia Volume 165, 15 July 2023 (Guest Editor)

 

Nagaishi Y, Murata D, Yoshizato H, Nonaka T, Itoh M, Hara H and Nakayama K
" Scaffold-free human vascular calcification model using a bio-three-dimensional printer"
Biofabrication 2023 Volume 15, Number 4

 

Zujir D, Al-Akashi Z, Nakamura A, Zhao C, Takahashi K, Aritomi S, Theoputra W, Kamiya D, Nakayama K and Ikeya M,
" Enhanced chondrogenic differentiation of iPS cell-derived mesenchymal stem/stromal cells via neural crest cell induction for hyaline cartilage repair"
Frontiers in Cell and Developmental Biology 2023 May10; 11:1140717 2023

 

Takagi K, Taniguchi D, Machino R, Uchida F, Hara R, Oishi K, Yamane Y, Iwatake M, Eguchi M, Mochizuki Y, Nakayama K and Nagayasu T
" Regeneration of the ureter using a scaffold-free live-cell structure created with the bio-three-dimensional printing technique"
Acta Biomaterialia Acta Biomaterialia,Volume 165,2023,Pages 102-110,ISSN 1742-7061

 

2022

Nonaka T, Nagaishi Y, Murata D, Hara H and Nakayama K
" 3D Printing in Nephrology"
In: Bezerra da Silva Junior, G., Nangaku, M. (eds) Innovations in Nephrology. Springer, Cham. 2022 https://doi.org/10.1007/978-3-031-11570-7_9 (Chapter)

 

Amamoto S, Itoh M, Takahashi B, Kitsuka T, Uchiashi K, Murata D, Node K, Nakayama K and Kamohara K
" Involvement of the PI3K/AKT Pathway in the Formation and Fusion of Spheroids Derived from Human Dermal Fibroblast for Tissue Engineering Technology"
Cell and Tissue Biology 16, pages 312–329 (2022)

 

Ikeguchi R, Aoyama T, Noguchi T, Ando M, Yoshimoto K, Sakamoto D, Iwai T, MIyazaki Y, Akieda S, Ikeya M, Nakayama K and Matsuda S
" Nerve regeneration using the Bio 3D conduit fabricated with spheroids"
Journal of Artificial Organs volume 25, pages289–297 (2022) (Review)

 

Fujimoto R, Murata D and Nakayama K
" Bio-3D printing of scaffold-free osteogenic and chondrogenic constructs using rat adipose-derived stromal cells"
Front. Biosci. (Landmark Ed) 2022 , 27(2), 052; https://doi.org/10.31083/j.fbl2702052

 

Kawai Y, Tohyama S, Arai K, Tamura T, Soma Y, Fukuda K, Shimizu H, Nakayama K and Kobayashi E
" Scaffold-Free Tubular Engineered Heart Tissue From Human Induced Pluripotent Stem Cells Using Bio-3D Printing Technology In Vivo"
Front Cardiovasc Med. 2022 Jan 20;8:806215. doi: 10.3389/fcvm.2021.806215. eCollection 2021.

 

Murata D, Ishikawa S, Sunaga T, Saito Y, Sogawa T, Tuji K, Shigenobu K, Nakayama K, Hobo S and Hatazoe T
" A preliminary study of osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem"
BMC Veterinary Research, 18, Article number: 53 (2022)

 

Ichikawa A, Neo S, Nukui R, Yamada Y,Nitta S, Iwaki H, Yanagi Y, Nakayama K, Sato S, Tateishi S and Hisasue M
"Establishment of large canine hepatocyte spheroids by mixing vascular endothelial cells and canine adipose-derived mesenchymal stem cells"
Regenerative Therapy, Volume 19, March 2022, Pages 1-8

 

2021

Moriyama M, Matsumoto K, Taniguchi D, Machino R, Tsuchiya T, Nakayama K and Nagayasu T
"Successful use of bio plugs for delayed bronchial closure after pneumonectomy in experimental settings"
Interactive CardioVascular and Thoracic Surgery, ivab306, November 2021
DOI https://doi.org/10.1093/icvts/ivab306

 

Nakamura A, Murata D, Fujimoto R, Tamaki S, Nagata S, Ikeya M,Toguchida J and Nakayama K
"Bio-3D printing iPSCs-derived human chondrocytes for articular cartilage regeneration"
Biofabrication, 13 044103 2021
DOI https://doi.org/10.1088/1758-5090/ac1c99

 

Arai K, Murata D, Takao S and Nakayama K
"Fabrication of Cardiac Constructs Using Bio-3D Printer"
In:Yoshida,Y editor. Pluripotent Stem-Cell Derived Cardiomyocytes, Springer US
DOI https://doi.org/10.1007/978-1-0716-1484-6
ISBN 978-1-0716-1483-9 (Chapter)

 

Arai K, Kitsuka T and Nakayama K
"Scaffold-based and scaffold-free cardiac constructs for drug testting"
Biofabrication 13 042001 2021 , DOI https://doi.org/10.1088/1758-5090/ac1257 (review)

 

Ando M, Ikeguchi R, Aoyama T, Tanaka M, Noguchi T, Miyazaki Y,Akieda S, Nakayama K, and Matsuda S
"Long-term Outcome of Sciatic Nerve Regeneration Using Bio3D Conduit Fabricated from Human Fibroblasts in a Rat Sciatic Nerve Model"
Cell Transplantation Volume: 30 Article first published online: June 9, 2021; Issue published: January 1, 2021

 

Hamada T, Nakamura A, Soyama A, Sakai Y, Miyoshi T, Yamaguchi S, Hidaka M, Hara T, Kugiyama T, Takatsuki M, Kamiya A, Nakayama K, and Eguchia S
"Bile duct reconstruction using scaffold-free tubular constructs created by Bio-3D Printer"
Regenerative therapy. Volume 16, March 2021, Pages 81-89

 

Shimoto T, Teshima C, Watanabe T, Zhang XY, Ishikawa A,Higaki H, and Nakayama K
"Study on Pipetting Motion Optimization of Automatic Spheroid Culture System for Spheroid Formation"
Journal of Robotics and Mechatronics. 33 (1), 78-87 2021

 

Arai K,and Nakayama K
" Bio-3D Printed Organs as Drug Testing Tools"
In: Nakayama K. (ed) Kenzan Method for Scaffold-Free Biofabrication. P 149-164 Springer Nature. 2021 https://doi.org/10.1007/978-3-030-58688-1_12 (Chapter)

 

Murata D
" Scaffold-Free Biofabrication for Articular Cartilage (and Subchondral Bone)"
In: Nakayama K. (ed) Kenzan Method for Scaffold-Free Biofabrication. P 149-164 Springer Nature. 2021 https://doi.org/10.1007/978-3-030-58688-1_12 (Chapter)

 

Nakayama K (Ed.)
"Kenzan Method for Scaffold-Free Biofabrication"
Springer Nature
2021. DOI https://doi.org/10.1007/978-3-030-58688-1
Book ISBN 978-3-030-58688-1

 

2020

Yurie H, Ikeguchi R, Aoyama T, Ito A, Tanaka M, Oda H,Noguchi T, Takeuchi H, Mitsuzawa S, Ando M, Yoshimoto K, Akieda S, Nakayama K, Matsuda S.
"Mechanism of Peripheral Nerve Regeneration Using a Bio 3D Conduit Derived from Normal Human Dermal Fibroblasts"
J Reconstr Microsurg 2021; 37(04): 357-364 DOI: 10.1055/s-0040-1716855

 

Yurie H, Ikeguchi R, Aoyama T, Tanaka M, Oda H, Takeuchi H, Mitsuzawa S, Ando M, Yoshimoto K, Noguchi T, Akieda S, Nakayama K, Matsuda S.
"Bio 3D Conduits Derived from Bone Marrow Stromal Cells Promote Peripheral Nerve Regeneration "
Cell TransplantationVolume 29, 1-9, 2020

 

Mitsuzawa S, Zhao C, Ikeguchi R, Aoyama T, Kamiya D, Ando M, Takeuchi H, Akieda S, Nakayama K, Matsuda S, Ikeya M
"Pro-angiogenic scaffold-free Bio three-dimensional conduit developed from human induced pluripotent stem cell–derived mesenchymal stem cells promotes peripheral nerve regeneration"
Scientific Reports volume 10, Article number: 12034 (2020)

 

Murata D, Fujimoto R, Nakayama K
"Osteochondral regeneration using adipose tissue derived-mesenchymal stem cells."
Int. J. Mol. Sci. 2020, 21(10), 3589 (review)

 

Arai K, Murata D, Takao S, Nakamura A, Itoh M,Kitsuka T, Nakayama K
"Drug response analysis for scaffold-free cardiac constructs fabricated using bio-3D printer "
Scientific Reports volume 10, Article number: 8972 (2020)

 

Murata D, Kunitomi Y, Harada K, Tokunaga S, Takao S, Nakayama K
"Osteochondral regeneration using scaffold-free constructs of adipose tissue-derived mesenchymal stem cells made by a bio three-dimensional printer with a needle-array in rabbits."
Regenerative Therapy, Volume 15, December 2020, Pages 77-89

 

Arai K, Murata D, Takao S, Verissiomo A, Nakayama K
"Cryopreservation method for spheroids and fabrication of scaffold-free tubular constructs "
PLoS One, 2020 Apr 2;15(4):e0230428

 

Murata D, Arai K, Nakayama K
"Scaffold-free Bio-3D Printing using Spheroids as ‘Bio-inks’ for Tissue (Re-)construction and Drug Response Tests"
Adv. Healthcare Mater., May 2020 (review)

 

Taniguchi D, Matsumoto K,Machino R, Takeoka Y, Elgalad A, Taura Y, Oyama S, Tetsuo T, Moriyama M, Takagi K, Kunizaki M, Tsuchiya M, Miyazaki M, Hatachi G, Matsuo N, Nakayama K, Nagayasu T
"Human lung microvascular endothelial cells as potential alternatives to human umbilical vein endothelial cells in bio-3D-printed trachea-like structures
Tissue and cell Volume 63, April 2020, 101321

 

Takeuchi H, Ikeguchi R, Aoyama T, Oda H, Yurie H, Mitsuzawa S, Tanaka M, Ohta S, Akieda S, Miyazaki Y, Nakayama K, Matsuda S.
"A scaffold-free Bio 3D nerve conduit for repair of a 10-mm peripheral nerve defect in the rats. "
Microsurgery , 40 (2), 207-216 Feb 2020

 

2019

Kitsuka T, Itoh M, Amamoto S, Arai K, Oyama J, Node K, Toda S, Morita S, Nishida T, Nakayama K
" 2-Cl-C.OXT-A Stimulates Contraction through the Suppression of Phosphodiesterase Activity in Human Induced Pluripotent Stem Cell-derived Cardiac Organoids"
PLoS ONE 14(7): e0213114, 2019

 

Mitsuzawa S, Ikeguchi R, Aoyama T, Takeuchi H, Yurie H, Oda H, Ohta S, Ushimaru M, Itho T , Tanaka M, Kunitomi Y, Tsuji M , Akieda S, Nakayama K, Matsuda S
"The efficacy of a scaffold-free Bio 3D conduit developed from autologous dermal fibroblasts on peripheral nerve regeneration in a canine ulnar nerve injury model : a preclinical proof of concept study"
Cell Transplantation Vol 28, Issue 9-10, 2019

 

Yamasaki A, Kunitomi Y, Murata D, Sunaga T, Kuramoto T, Sogawa T, Misumi K
"Osteochondral regeneration using constructs of mesenchymal stem cells made by bio three-dimensional printing in mini-pigs"
J Orthop Res. 2019 Jun;37(6):1398-1408

 

Itoh M, Mukae Y, Kitsuka T, Arai K, Nakamura A, Uchihashi K, Toda S, Matsubayashi K, Oyama J, Node K, Kami D, Gojo S, Morita S, Nishida T, Nakayama K*, and Kobayashi E* _{*Co-corresponding author}
"Development of an immunodeficient pig model allowing long-term accommodation of artificial human vascular tubes"
Nature Communications 2019 May 21;10(1):2244.

 

Nakanishi Y, Okada T, Takeuchi N, Kozono N, Senjyu T, Nakayama K, Nakashima Y
"Histological evaluation of tendon formation using a scaffold-free three-dimensional-bioprinted construct of human dermal fibroblasts under in vitro static tensile culture"
Regenerative Therapy Volume 11, December 2019, Pages 47-55

 

Taguchi T, Yanagi Y, Yoshimaru K, Zhang X, Matsuura T, Nakayama K, Kobayashi K, Yamaza H, Nonaka K, Ohga S
"Regenerative medicine using stem cells from human exfoliated deciduous teeth (SHED): a promising new treatment in pediatric surgery."
Surgery today 49 (4), 316-322

 

Takeoka Y, Matsumoto K, Taniguchi D, Tsuchiya T, Machino R, Moriyama M, Oyama S, Tomoyuki T, Taura Y, Takagi K, Yoshida T,Matsuo N, Abdelmotagaly Elgalad, Kunizaki M,Tobinaga S, Nonaka T, Hidaka S,Yamasaki N, Nakayama K, Nagayasu T
"Regeneration of esophagus using a scaffold-free biomimetic structure created with biothree-dimensional printing."
PLoS ONE March 14(3): e021133

 

Machino R, Matsumoto K, Taniguchi D, Tsuchiya T, Takeoka Y, Taura Y, Moriyama M, Tetsuo T, Oyama S, Takagi K, Miyazaki T, Hatachi G, Doi R, Shimoyama K, Matsuo N, Yamasaki N, Nakayama K, Nagayasu T
"Replacement of Rat Tracheas by Layered, Trachea-like, Scaffold-free Structures of Human Cells Using Bio-3D Printing System."
Adv. Healthcare Mater., 2019, 1800983

 

Oshima T, Nakase J, Toratani T, Numata H, Takata Y, Nakayama K, Tsuchiya H
"A Scaffold-Free Allogeneic Construct From Adipose-Derived Stem Cells Regenerates an Osteochondral Defect in a Rabbit Model"
Arthroscopy: The Journal of Arthroscopic & Related Surgery 35 (2), 583-593

 

2018

Arai K, Murata D, Ana R.Verissimo, Mukae Y, Itoh M, Nakamura A, Morita S and Nakayama K.
"Fabrication of scaffold-free tubular cardiac constructs using a bio-3D printer, "
PLoS ONE Decmber 13(12): e0209162. 

 

Mukae Y, Itoh M, Noguchi R, Furukawa K, Arai KI, Oyama JI, Toda S, Nakayama K, Node K, Morita S.
"The addition of human iPS cell-derived neural progenitors changes the contraction of human iPS cell-derived cardiac spheroids."
Tissue Cell. 2018 Aug;53:61-67. doi: 10.1016/j.tice.2018.05.002. Epub 2018 May 5.

 

Murata D,Akieda S,Misumi K, Nakayama K.
"Osteochondral regeneration with a scaffold-free three-dimensional construct of adipose tissue-derived mesenchymal stromal cells in pigs."
Tissue Engineering and Regenerative Medicine February 2018,15(1)101-113

 

Taniguchi D, Matsumoto K, Tsuchiya T, Machino R, Takeoka Y, Elgalad A, Gunge K, Takagi K, Taura Y, Hatachi G, Matsuo N, Yamasaki N, Nakayama K, Nagayasu T.
"Scaffold-free trachea regeneration by tissue engineering with bio-three-dimensional printing"
Interactive Cardiovascular and Thoracic Surgery, April 2018,26(4)713

 

Xiu-Ying Zhang, Yanagi Y, Zijing Sheng, Nagata K, Nakayama K, Taguchi T.
"Regeneration of diaphragm with bio-3D cellular patch"
Biomaterials June 2018, 167:1-14      

 

J Groll, J A Burdick, D-W Cho, B Derby, M Gelinsky, S C Heilshorn, T Jüngst, J Malda, V A Mironov,  Nakayama K, A Ovsianikov, W Sun,Takeuchi S, J J Yoo, T B F Woodfield
"A definition of bioinks and their distinction from biomaterial inks"
Biofabrication 11 013001 (Perspective)

 

2017

Yurie H, Ikeguchi R, Aoyama T, Kaizawa Y, Tajino J, Ito A, Ohta S, Oda H, Takeuchi H, Akieda S, Tsuji M, Nakayama K, Matsuda S.
"The efficacy of a scaffold-free bio 3D conduit developed from human fibroblasts on peripheral nerve regeneration in a rat sciatic nerve model "
PLoS One February 13,2017,12(2): e0171448

 

Toratani T, Nakase J, Numata H, Oshima T, Takata Y, Tsuchiya H, Nakayama K.
"Scaffold-free tissue-engineered allogenic adipose-derived stem cells promote meniscus healing."　
Arthroscopy:The Journal of Arthroscopic and Related Surgery, February 2017,33(2):346-354

 

Moldovan N.I., Hibino N, Nakayama K.
"Principles of the 'Kenzan' method for robotic cell spheroid-based three-dimensional bioprinting."
Tissue Engineering Part B:Reviews,June 2017,23(3)237-244 (Review)


Yanagi Y, Nakayama K, Taguchi T, Enosawa S, Tamura T, Yoshimaru K, Matsuura T, Hayashida M, Kohashi K, Oda Y, Yamaza T, Kobayashi E.
"In vivo and ex vivo methods of growing a liver bud through tissue connection"
Scientific Reports October 26 2017,7(1)14085

 

Verissimo A.R., Nakayama K.
"Scaffold-Free Biofabrication. "
3D Printing and Biofabrication. Reference Series in Biomedical Engineering. Springer (Chaptor)

 

2016

Noguchi R, Nakayama K, Itoh M, Kamohara K, Furukawa K, Oyama J, Node K, Morita S.
"Development of a three-dimensional pre-vascularized scaffold-free contractile cardiac patch for treating heart disease."
The Journal of Heart and Lung Transplantation
January 2016, 35(1),137-145

 

2015

Murata D, Tokunaga S, Tamura T, Kawaguchi H, Miyoshi N, Fujiki M, Nakayama K,
Misumi K.
"A preliminary study of osteochondral regeneration using a scaffold-free three-dimensional construct of porcine adipose tissue-derived mesenchymal stem cells. "
Journal of Orthopaedic Surgery and Research 2015 Mar. 18;10:35

 

 

Itoh M, Nakayama K, Noguchi R, Kamohara K, Furukawa K, Uchihashi K, Toda S, Oyama J,  Node K, Morita S.
"Scaffold-free tubular tissues created by a Bio-3D printer undergo remodeling and endothelialization when implanted in rat aortae."
PLOS ONE September 1, 2015, 10(9): e0136681

 

2014

Murata D, Miyakoshi D, Hatazoe T, Miura N, Tokunaga S, Fujiki M, Nakayama K, Misumi K.
"Multipotency of equine mesenchymal stem cells derived from synovial fluid."
The Veterinary Journal, 2014 Oct, 202(1):53-61

 

Ishihara K, Nakayama K, Akieda S, Matsuda S, Iwamoto Y.
"Simultaneous regeneration of full-thickness cartilage and subchondral bone defects in vivo using a three-dimensional scaffold-free autologous construct derived from high-density bone marrow-derived mesenchymal stem cells"
Journal of Orthopaedic Surgery and Research 2014 Oct. 14;9:98

 

2013

K Nakayama
"In Vitro Biofabrication of Tissues and Organs"
Biofabrication: Micro-and Nano-Fabrication. Printing Patterning and Assemblies. (Chapter)

Shimoto T, Hidaka N, Sasaki H, Nakayama K, Akieda S, Matsuda S, Miura H, Iwamoto Y.
"Bio rapid prototyping project: Development of spheroid formation system for regenerative medicine. "
Information Technology Convergence, 2013, 253(Lecture Notes in Electrical Engineering):855-862 

2012

Shimoto T, Nakayama K, Matsuda S, Iwamoto Y.
"Building of HD MACs using cell processing robot for cartilage regeneration. "
Journal of Robotics and Mechatronics 2012, 24(2):347-353

 

2009

Akasaki Y, Matsuda S, Nakayama K, Fukagawa S, Miura H, Iwamoto Y.
"Mevastatin reduces cartilage degradation in rabbit experimental osteoarthritis through inhibition of synovial inflammation."
Osteoarthritis Cartilage. 2009 Feb;17(2):235-243. 

 

Yoshida T, Sakamoto A., Tsukamoto N., Nakayama K., Iwamoto Y
"Establishment of an animal model of a pasteurized bone graft, with a preliminary analysis of muscle coverage or FGF-2 administration to the graft. "
Journal of Orthopaedic Surgery and Research 2009 Aug 4;4:31

 

2005

Nakayama K, Matsuda S, Miura H, Iwamoto Y, Higaki H, Otsuka K.
"Contact stress at the post-cam mechanism in posterior-stabilised total knee arthroplasty."
The Bone & Joint Journal (formerly known as Journal of Bone and Joint Surgery British Volume)
2005 Apr; 87-B(4):483-488